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Image Search Results
Journal: STAR Protocols
Article Title: Analyzing the topology of N-linked glycans by PNGase F accessibility assay
doi: 10.1016/j.xpro.2023.102458
Figure Lengend Snippet:
Article Snippet:
Techniques: Purification, Recombinant, Protease Inhibitor, Saline, Transfection, Plasmid Preparation, Software, Sterility, Blocking Assay, Cell Culture, Western Blot
Journal: Theranostics
Article Title: Hepatomas are exquisitely sensitive to pharmacologic ascorbate (P-AscH - )
doi: 10.7150/thno.35378
Figure Lengend Snippet: The effect of ascorbate and H 2 O 2 on hepatic cells. Relative cytotoxicity of ascorbate (A, C) and H 2 O 2 (B) on liver normal and cancer cells. Ascorbate induced G1 arrest (D-F) and apoptosis (I-K) in Huh-7 cells. qRT-PCR analysed CDK4 (G) and CDK6 (H), c-Myc (L), and Casp3 (M) gene expression in ascorbate-treated Huh-7 cells. Immunoblots analysis of c-Myc and Casp3 expression in ascorbate-treated Huh-7 cells (N).
Article Snippet: The membranes were blocked with 5% milk in PBST and then incubated with a rabbit anti-Glut1 (Cat # : D160433, BBI Solutions) or rabbit anti-Glut 3 (Cat # : D260435, BBI Solutions), rabbit anti-human Casp 3 (Cat # : D220074, BBI Solutions.) or
Techniques: Quantitative RT-PCR, Expressing, Western Blot